Next-generation sequencing (NGS) libraries can be quantified with minimal sample handling and without the need to generate a standard curve using digital PCR. This method enables accurate and precise library quantification, a critical step in both the Ion Torrent™ and Illumina® workflows, allowing for maximizing sequencing yields downstream. To achieve this high degree of precision, a TaqMan® Assay, designed to span both the forward and reverse adapters specific to each library, is available.
This approach limits quantification to library constructs that contain both adapter sequences. Ultimately, using digital PCR to quantify NGS libraries decreases overall sequencing costs by ensuring an accurate quantification upfront, minimizing the need to re-run or repeat sequencing of samples.
See demonstrated protocols on the QuantStudio® 3D Digital PCR System for both NGS platforms: